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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agroenergia. Para informações adicionais entre em contato com cnpae.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Agroenergia. |
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Data corrente: |
24/11/2020 |
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Data da última atualização: |
24/11/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
SANTOS, L. L.; SALGADO, J. A.; DRUMMOND, M. G.; BASTIANETTO, E.; SANTOS, C. P.; BRASIL, B. dos S. A. F.; TACONELI, C. A.; OLIVEIRA, D. A. A. |
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Afiliação: |
Lívia L. Santos, Universidade Federal de Minas Gerais; Jordana A. Salgado, Universidade Estadual do Norte Fluminense Darcy Ribeiro; Marcela G. Drummond, Myleus Biotecnologia; Eduardo Bastianetto, Universidade Federal de Minas Gerais; Clóvis P. Santos, Universidade Estadual do Norte Fluminense Darcy Ribeiro; BRUNO DOS SANTOS A F BRASIL, SPD; Cesar A. Taconeli, Universidade Federal do Paraná; Denise A. A. Oliveira, Universidade Federal de Minas Gerais. |
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Título: |
Molecular method for the semiquantitative identification of gastrointestinal nematodes in domestic ruminants. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Parasitology Research, v. 119, p. 529-543, Feb. 2020. |
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DOI: |
https://doi.org/10.1007/s00436-019-06569-3 |
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Idioma: |
Inglês |
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Conteúdo: |
Standard diagnostic methods currently in use for the identification of helminth infections in ruminants are based on the morphological analysis of immature and adult stages of parasites. This paper describes a method for the semiquantitative identification of nematodes, mainly Trichostrongyloidea, at species-level resolution. The method is based on amplification and fragment analysis followed by minisequencing of the ITS-2 region (internal transcribed spacer 2) of the ribosomal DNA of parasite eggs or larvae. This method allows for the identification of seven genera (Chabertia, Cooperia, Haemonchus, Oesophagostomum, Ostertagia, Teladorsagia, and Trichostrongylus) and 12 species (Chabertia ovina, Cooperia curticei, Cooperia punctata, Cooperia oncophora/Cooperia surnabada, Haemonchus contortus, Haemonchus placei, Haemonchus longistipes, Oesophagostomum asperum, Oesophagostomum radiatum, Ostertagia ostertagi, Trichostrongylus axei, and Trichostrongylus colubriformis) of infectious nematodes of domestic ruminants. The concordance between the morphological and molecular analyses in the detection of genera ranged from 0.84 to 0.99, suggesting the proposed detection method is specific, semiquantitative, less laborious, and highly cost-efficient. |
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Palavras-Chave: |
ITS-2; SNaPshot minisequencing. |
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Thesagro: |
Nematóide; Parasito de Animal; Parasitose; Ruminante. |
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Thesaurus NAL: |
Gastrointestinal nematodes; Ruminants; Veterinary parasitology. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02249naa a2200325 a 4500
001 2127014
005 2020-11-24
008 2020 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s00436-019-06569-3$2DOI
100 1 $aSANTOS, L. L.
245 $aMolecular method for the semiquantitative identification of gastrointestinal nematodes in domestic ruminants.$h[electronic resource]
260 $c2020
520 $aStandard diagnostic methods currently in use for the identification of helminth infections in ruminants are based on the morphological analysis of immature and adult stages of parasites. This paper describes a method for the semiquantitative identification of nematodes, mainly Trichostrongyloidea, at species-level resolution. The method is based on amplification and fragment analysis followed by minisequencing of the ITS-2 region (internal transcribed spacer 2) of the ribosomal DNA of parasite eggs or larvae. This method allows for the identification of seven genera (Chabertia, Cooperia, Haemonchus, Oesophagostomum, Ostertagia, Teladorsagia, and Trichostrongylus) and 12 species (Chabertia ovina, Cooperia curticei, Cooperia punctata, Cooperia oncophora/Cooperia surnabada, Haemonchus contortus, Haemonchus placei, Haemonchus longistipes, Oesophagostomum asperum, Oesophagostomum radiatum, Ostertagia ostertagi, Trichostrongylus axei, and Trichostrongylus colubriformis) of infectious nematodes of domestic ruminants. The concordance between the morphological and molecular analyses in the detection of genera ranged from 0.84 to 0.99, suggesting the proposed detection method is specific, semiquantitative, less laborious, and highly cost-efficient.
650 $aGastrointestinal nematodes
650 $aRuminants
650 $aVeterinary parasitology
650 $aNematóide
650 $aParasito de Animal
650 $aParasitose
650 $aRuminante
653 $aITS-2
653 $aSNaPshot minisequencing
700 1 $aSALGADO, J. A.
700 1 $aDRUMMOND, M. G.
700 1 $aBASTIANETTO, E.
700 1 $aSANTOS, C. P.
700 1 $aBRASIL, B. dos S. A. F.
700 1 $aTACONELI, C. A.
700 1 $aOLIVEIRA, D. A. A.
773 $tParasitology Research$gv. 119, p. 529-543, Feb. 2020.
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